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ApexBio zdevd-fmk
N27 dopaminergic cells were pre-treated with either 20 or 50 μM of <t>the</t> <t>caspase-3</t> inhibitor zDEVD-fmk before exposure to 300 μM of Mn for 24 h in reduced sera. Expression of ΔNp73 protein was measured by Western blot assays (A). Densitometric analysis was performed (B). Data are expressed as percent of control and represented as mean ± S.E.M. from three independent experiments. Asterisks (*, p < 0.05; **, p<0.01) indicate significant differences between treatment and control groups.
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Becton Dickinson caspase-3 inhibitor (benzyloxycarbonyl-devd-aldehyde
N27 dopaminergic cells were pre-treated with either 20 or 50 μM of <t>the</t> <t>caspase-3</t> inhibitor zDEVD-fmk before exposure to 300 μM of Mn for 24 h in reduced sera. Expression of ΔNp73 protein was measured by Western blot assays (A). Densitometric analysis was performed (B). Data are expressed as percent of control and represented as mean ± S.E.M. from three independent experiments. Asterisks (*, p < 0.05; **, p<0.01) indicate significant differences between treatment and control groups.
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Cayman Chemical z-devd-fmk
N27 dopaminergic cells were pre-treated with either 20 or 50 μM of <t>the</t> <t>caspase-3</t> inhibitor zDEVD-fmk before exposure to 300 μM of Mn for 24 h in reduced sera. Expression of ΔNp73 protein was measured by Western blot assays (A). Densitometric analysis was performed (B). Data are expressed as percent of control and represented as mean ± S.E.M. from three independent experiments. Asterisks (*, p < 0.05; **, p<0.01) indicate significant differences between treatment and control groups.
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Image Search Results


N27 dopaminergic cells were pre-treated with either 20 or 50 μM of the caspase-3 inhibitor zDEVD-fmk before exposure to 300 μM of Mn for 24 h in reduced sera. Expression of ΔNp73 protein was measured by Western blot assays (A). Densitometric analysis was performed (B). Data are expressed as percent of control and represented as mean ± S.E.M. from three independent experiments. Asterisks (*, p < 0.05; **, p<0.01) indicate significant differences between treatment and control groups.

Journal: Neurotoxicology

Article Title: p73 gene in Dopaminergic Neurons is Highly Susceptible to Manganese Neurotoxicity

doi: 10.1016/j.neuro.2016.04.012

Figure Lengend Snippet: N27 dopaminergic cells were pre-treated with either 20 or 50 μM of the caspase-3 inhibitor zDEVD-fmk before exposure to 300 μM of Mn for 24 h in reduced sera. Expression of ΔNp73 protein was measured by Western blot assays (A). Densitometric analysis was performed (B). Data are expressed as percent of control and represented as mean ± S.E.M. from three independent experiments. Asterisks (*, p < 0.05; **, p<0.01) indicate significant differences between treatment and control groups.

Article Snippet: We purchased the tetrapeptide caspase-3 inhibitor zDEVD-fmk from ApexBio (Houston, TX).

Techniques: Expressing, Western Blot